Specification location: AQA GCSE Biology Paper 1, Section 4.1 - Cell biology
Summary of method: Small pieces of potato (or other vegetables) are cut and their masses are recorded. They are then immersed in a variety of strengths of sugar or salt solutions and left for osmosis to occur (an absolute minimum of 15 minutes). They are then removed and their exteriors are dried. Their masses are then measured again so that the change in their mass and their percentage change in mass can be calculated. From this, the approximate concentration inside the potato cells can be determined by plotting the results on a graph and looking for the point at which the line of best fit crosses the x-axis (no change in mass means that the solution is isotonic).
For more detail on the set-up of this experiment, watch our high-quality required practical tutorial on YouTube.
Osmosis required practical variables:
Independent variable: Concentration of sugar or salt solution.
Dependent variable: Percentage mass change of potato pieces (or length change of potato pieces)
Control variables: Length, surface area, temperature, potato age and type, time
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Sample osmosis required practical results:
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Source: AQA GCSE Biology (9-1) required practical handbook
AQA GCSE Science subject-specific terminology:
- As students will be measuring small changes in mass, probably with a two-decimal-place balance, this is an ideal opportunity to discuss the idea of resolution.
- As the concentrations of the solutions will vary, this is also an ideal opportunity to discuss both the ideas of both intervals and range.
Other blog posts in our AQA GCSE Biology (9-1) Required Practical series:
Getting the most out of GCSE Biology Required Practicals: Microscopy
Getting the most out of GCSE Biology Required Practicals: Microbiology
Getting the most out of GCSE Biology Required Practicals: Food tests
Getting the most out of GCSE Biology Required Practicals: Enzyme activity
Getting the most out of GCSE Biology Required Practicals: Photosynthesis
Getting the most out of GCSE Biology Required Practicals: Reaction times
Getting the most out of GCSE Biology Required Practicals: Plant responses
Getting the most out of GCSE Biology Required Practicals: Field Investigations
Getting the most out of GCSE Biology Required Practicals: Decay